Journal: Endocrinology

Article Title: Short-Term High-Fat Diet Increases Leptin Activation of CART Neurons and Advances Puberty in Female Mice

doi: 10.1210/en.2017-00452

Figure Lengend Snippet: Antibody Table

Article Snippet: DYN , SQENPNTYSEDLDV; corresponding to residues 235–248 of rat prodynorphin , , Neuromics, GP10110 , Guinea pig; polyclonal , 1:250 , AB_2162536.

Techniques: Sequencing

Journal: Neurobiology of Stress

Article Title: A projection from the paraventricular nucleus of the thalamus to the shell of the nucleus accumbens contributes to footshock stress-induced social avoidance

doi: 10.1016/j.ynstr.2020.100266

Figure Lengend Snippet: Number of double-labeled cFos neurons in the NAcSh expressing opioid neuropeptides following the social approach-avoidance test. (A) For dynorphin (DYN), the number of cFos+/DYN + double-labeled NAcSh neurons per mm 2 . (B) For enkephalin (ENK), the number of cFos+/ENK + double-labeled NAcSh neurons per mm 2 . (C) Representative images of the NAcSh showing cFos+ (green) and ENK+ (blue), and DYN+ (red) neurons in an hM4Di + Sal and an hM4Di + Clz case. Data are means ± SEM. ** p < 0.01. Group size n = 4 cases for each bar. Scale bars, 25 and 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Sections of the NAcSh were incubated in primary antibodies against ENK (rabbit anti-preproenkephalin; 1:1000; E-3220-24, USBiological, Salem, MA, USA) and DYN (guinea pig anti-preprodynorphin; 1:2000; GP10110, Neuromics, Edina, MN, USA) in addition to mouse anti-cFos primary antibody (1:500; ab208942, Abcam, Cambridge, UK) overnight.

Techniques: Labeling, Expressing

Journal: Nature communications

Article Title: eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference

doi: 10.1038/ncomms8194

Figure Lengend Snippet: ( a ) Proteins co-immunoprecipitated with Flag-HA-Ago2 from HEK293 cells were separated by SDS-PAGE (silver staining) and analyzed by mass spectrometry. eIF1A is a novel Ago2 interacting protein (*:detected peptides of eIF1A). ( b ) Fluorescence immunostaining was performed with anti-eIF1A (monoclonal) and anti-Ago2 (monoclonal) antibodies and DAPI (4′,6-diamidino-2-phenylindole) in HEK293 and human glioblastoma multiforme T98G cells. White arrows show the co-localized eIF1A and Ago2. ( c ) eIF1A interacts with Ago2. Human eIF1A was transiently overexpressed in stable HEK293 cells stably expressing Agos (1~4). Immunoprecipitation assays were performed with anti-eIF1A (monoclonal) antibody and immuno blotting (IB) with anti-Agos antibodies in the upper panel and anti-eIF1A antibodies in the bottom panel.

Article Snippet: The Ago2 interacted proteins were immunoprecipitated by anti-HA monoclonal antibodies (Cell Signaling, 2367S, 20μl for immunoprecipitation assay) immobilized to Dyna beads.

Techniques: Immunoprecipitation, SDS Page, Silver Staining, Mass Spectrometry, Fluorescence, Immunostaining, Bioprocessing, Stable Transfection, Expressing

Journal: Endocrinology

Article Title: Short-Term High-Fat Diet Increases Leptin Activation of CART Neurons and Advances Puberty in Female Mice

doi: 10.1210/en.2017-00452

Figure Lengend Snippet: Antibody Table

Article Snippet: Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog No., and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used RRID NKB Rabbit anti-NKB Bachem, Peninsula Laboratories, T-4450 Rabbit; polyclonal 1:2500 AB_518485 DYN SQENPNTYSEDLDV; corresponding to residues 235–248 of rat prodynorphin Neuromics, GP10110 Guinea pig; polyclonal 1:250 AB_2162536 p-STAT3 (Tyr705) p-STAT3 (Tyr705) antibody Cell Signaling Technology, 9131 Rabbit; polyclonal 1:1000 AB_331586 CART CART antibody (N-20) Santa Cruz Biotechnology, sc-18068 Goat; polyclonal 1:500 AB_2068572 Rabbit IgG Biotin-SP-conjugated AffiniPure donkey anti-rabbit IgG (H+L) Jackson ImmunoResearch, 711-065-152 Donkey; polyclonal 1:500 AB_2340593 Guinea pig IgG (H+L) Biotin-SP (long spacer) AffiniPure donkey anti-guinea pig IgG (H+L) Jackson ImmunoResearch, 706-065-148 Donkey; polyclonal 1:500 AB_2341097 Rabbit IgG (H+L) Alexa Fluor ® 594 AffiniPure donkey anti-rabbit IgG (H+L) Jackson ImmunoResearch, 711-585-152 Donkey; polyclonal (1:250) AB_2340621 Goat IgG (H+L) Biotin-SP (long spacer) AffiniPure donkey anti-goat IgG (H+L) Jackson ImmunoResearch,705-065-003 Donkey; polyclonal (1:500) AB_2340396 Open in a separate window Abbreviations: AB, antibody; H, heavy chain; IgG, immunoglobulin G; L, light chain; RRID, Research Resource Identifier.

Techniques: Sequencing